Journal: Frontiers in Nutrition

Article Title: Shikimic acid attenuates oxidative stress-induced senescence in 3D4/21 cells by modulating PARP1 activity and DNA damage response

doi: 10.3389/fnut.2025.1614148

Figure Lengend Snippet: Effect of shikimic acid on XRCC1, PARP1 protein expression and PAR levels ( n = 3, where 3 is the number of biological replicates per group). (A) Representative Western blot images for XRCC1, PARP1, and PAR levels. (B) Effect of SA on XRCC1 protein expression in 3D4/21 cells. (C) Effect of SA on PARP1 protein expression in 3D4/21 cells. (D) Effect of SA on 3D4/21 cells PAR levels.

Article Snippet: Membranes were then incubated with primary antibodies against XRCC1 (Abcam, ab9147, Cambridge, UK), PARP1 (Affinity Biosciences, DF7198, Cincinnati, USA), and PAR (Cell Signaling Technology, CST 83732, Massachusetts, USA) at 4 °C for 12 h. Subsequently, membranes were incubated with appropriate secondary antibodies for 2 h at room temperature.

Techniques: Expressing, Western Blot